Enantiomeric Separation of 2-Hydroxyglutarate Using Chiral Mobile Phase Additives
نویسندگان
چکیده
2-Hydroxyglutarate (2-HG) was enantiomerically separated using copper(II) acetate and N,N-dimethyl-L-phenylalanine as chiral additives. These compounds formed diastereomeric complexes with 2-HG, which were successfully on an achiral (ODS) column. Several parameters, such additive concentration, the type of organic modifier, column temperature, optimized. Using optimal mobile phase (1 mM 2 in a 10% aqueous methanol solution), 2-HG enantiomers 15 min resolution 1.93.
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ژورنال
عنوان ژورنال: Chromatography
سال: 2022
ISSN: ['1342-8284', '1348-3315']
DOI: https://doi.org/10.15583/jpchrom.2021.016